Getting Started with ESTmapper
How To ESTmapper
Chapter 1: Quick Start
ESTmapper needs to pre-compute data structures on the entire genome. This is done via the configureESTmapper.pl command:
% perl ESTmapper/bin/configureESTmapper.pl \ -genome yeast_chr.fasta \ -genomedir yeast
Once that is done, any amount of sequence can be ESTmapped to that genome.
% perl ESTmapper/bin/ESTmapper.pl \ -genomedir yeast \ -outputdir yeastmap \ -mapest yeast_est.fasta \ -verbose -aligns
Hooray! You've just mapped yeast ESTs!
Chapter 2: Slower Start
Read the ESTmapper Documentation.